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Antimicrobial Properties in the Mucin of Different Species of Snails Identified by Using the Kirby Bauer Method

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Carmen L. Del Toro, Noeli Minaya and Ariela Ramírez

 

 Abstract

Antibiotics are central to the management of infectious diseases. The increasing resistance developed by bacteria to antibiotics represents a growing worldwide health and safety issue. There is a need to develop new antibiotics due to this serious problem. Antibiotics are natural or chemical agents that will destroy or inhibit bacterial growth. Some practices among people who work with snails suggest that their mucin has antimicrobial properties. In this research we wanted to investigate if the mucin of the snails tested had antimicrobial properties. We worked with three species of snails: Caracollus caracolla, Polidonte lima, and Caracollus marginella, all found in Yabucoa Puerto Rico. We used the standardized method: Kirby Bauer Method Disk Diffusion Test to study the antimicrobial susceptibility of six different bacterias to the mucin of each of these snails. The bacterias tested for the method were: Staphylococcus aureus, Escherichia coli, Pseudomona aeruginosa, Enterococcus faecalis, Bacillus subtillis and Micrococcus luteus. A 0.5 McFarland turbidity standard was used to standardize the inoculum density. To validate the accuracy of the procedure we used the following quality control organisms: Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Pseudomona aeruginosa ATCC 27853, Enterococcus faecalis, ATCC 29212. We prove thought experimentation that the mucin of Caracollus caracole have antimicrobial properties against Micrococcus luteus and could become a new antibiotic. In our research the mucin of Caracollus caracole prove to be an effective natural drug against infections caused by Micrococus luteus. M. luteus is a gram positive bacteria. These bacteria have been associated to immunocompromised patients causing them urinary tract infections and a case of meningitis.

 

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